Regulatory
pBad Weak

Part:BBa_K427000

Designed by: Tatiana J. Núñez Elizondo   Group: iGEM10_Tec-Monterrey   (2010-10-19)

PBad Weak Reverse

PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.

Usage and Biology

It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary.

This part has no special safety considerations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

BBa_K206001 part created by British Columbia team in 2009.

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Parameters
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